MHH-ES-1

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

MHH-ES-1 (human, sarcoma, Ewing's)

SPECIES: human
TUMOR: sarcoma, Ewing's

DSMZ catalog code: ACC 167
Morphology: adherent cells of variable size, single or in small clusters, growing as monolayer
Description: human Ewing's sarcoma established from the ascites of a 12-year-old Turkish boy with Ewing's sarcoma of the left pelvis (with peritoneal metastasis) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. T. Pietsch, Institute of Neuropathology, University of Bonn, Bonn, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture up to 1:10 every 2-3 days using trypsin/EDTA; cells grow rather fast at 37C with 5% CO2 about 25 x 106 cells/80 cm 2; doubling time of ca. 28 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human hyperdiploid karyotype with 12% polyploidy; 49(46-51)<2n>XXY, +X, +1, +8, +12, -17, dup(1) (q31.1q32.2), del(3)(p11), der(4)t(4;?6)(p14;p11), t(6;7)(q11;p11), der(8)t(8;11)(p12;q13), t(11;22)(q24;q12)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament- vimentin++) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)

Bibliographic references:

Cancer Genet 1989;38:167 (abstract)

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105