MH1C1

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

MH1C1 (rat, buffalo, liver, hepatoma)

SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma

DSMZ catalog code: ACC 106
Morphology: adherent cells growing in big clusters, different cellular forms
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: rat hepatoma a clonal strain of epithelial cells was derived from a transplantable Morris hepatoma ( 7795, Buffalo strain) in 1967; cells were described to synthesize and to secrete serum albumin and serum complement component C9 confirmed as rat with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. M. Ferro, Institute of General Pathology, University of Genova, Genova, Italy
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% Dulbecco's MEM + 10-15% FBS split confluent culture 1:2 to 1:4 about once or twice per week using trypsin/EDTA; seed out at ca. 2 x 106 cells/75 cm 2 at 37C with 5-10% CO2 cell harvest of about 20 x 106 cells/175 cm 2; doubling time of ca. 33-60 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid rat karyotype with 10% polyploidy; 44(40-46)<2n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of MPI, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 144, ECACC: 85112702, ICLC: ATL98014

Bibliographic references:

Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
Chem Biol Interact 1988;65:629-634
Exp Cell Res 1991;196:330-336 - PMID: 1716586
In Vitro 1970;6:32 - PMID: 4399803
J Cell Biol 1969;40:236-247 - PMID: 4177659
J Cell Res 1969;40:236
Science 1970;170:533 - PMID: 4908238