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SPECIES: human, Caucasian
ECACC catalog code: 95051030
Description: Human uterus sarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and chemotherapeutic agent studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:6 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm2 using 0.03% EDTA; 5% CO2; 37C. DO NOT USE TRYPSIN.
Karyotype: Modal No. 45, monosomic forms of chromosomes 5, 6 and 7
A human uterine sarcoma derived in 1980 from a tumour obtained at the time of a hysterectomy from a 56 year old Caucasian female. The cells form tumours in athymic nude mice, readily form colonies in soft agar and have a reported doubling time of 22-24 hours. The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine dacarbazine, cisplatin, melphalan, vincristine methotrexate and etoposide. The multi drug-resistant cell line MES-SA/Dx5 was established from MES-SA cells grown in the presence of doxorubicin.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1976, ICLC: HTL96008
- Cancer Res 1983;43:4943-4950 - PMID: 6883344
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.