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DSMZ catalog code: ACC 62
Morphology: epithelial-like adherent cells with long, narrow, often branching cell bodies
Description: human melanoma established from the primary tumor of a woman with melanoma in 1976; cells express mRNA of the oncogene c-myc confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, University of Munich, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% RPMI 1640 + 5-10% FBS split confluent culture 1:5 to 1:10 using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm 2 at 37C with 5% CO2 about 10-12 x 106 cells/80 cm 2;
doubling time of ca. 24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypertriploid with 1.5% polyploidy; 74(72-76)<3n>XX, -X, +3, +7, +7, -8, -10, -11, +15, -16, +17, +18, +20, +21, + m; del(1)(q42), del(3)(p21), i(7q)x2, del(9)(p13), der(15)t(15;?)(q21;?), idic17(q22-23), del(X)(q11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament-vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
- Int J Cancer 1988;41:542-547 - PMID: 3258589
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.