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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute megakaryoblastic
DSMZ catalog code: ACC 104
Morphology: single round cells in suspension, a few cells are lightly adherent
Description: human acute megakaryoblastic leukemia established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-ss, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. L. Pegoraro, University of Turin, Turin, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% Iscove's MDM or RPMI 1640 + 10-20% FBS + IL-3 (10 ng/ml) or GM-CSF (10 ng/ml) or 10-20% vol conditioned medium of cell line 5637 (DSM ACC 35) cells are difficult to culture! maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:3 every two days; cells grow slowly; seed out at about 4 x 105 cells/ml at 37C with 5% CO2 cell harvest of a. 0.5 x 106 cells/ml; maximal density at ca. 2 x 10 6 cells/ml;
doubling time of about 32-46 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near-diploid karyotype; 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3+ CD13+ CD14- CD15+ CD19- CD33+ CD34- CD41a- CD42b- GlyA-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ICLC: HTL04002
- Br J Haematol 1988;69:359-366 - PMID: 3261598
- J Cell Physiol 1990;145:458-464 - PMID: 2273055
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.