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SPECIES: human, Caucasian
ECACC catalog code: 94120801
Description: Human colon adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA)
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) (Heat inactivated). Split confluent cultures 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using trypsin 0.25% or trypsin/EDTA; 5% CO2, 37C.
Karyotype: Modal No. 63 (2n=46), hypotriploid
Established in 1974 from the second in a series of 3 primary colonic tumours resected from a 65 year old Caucasian female, which was classified as a Duke's B mucin producing colon adenocarcinoma. LS 123 is aneuploid, fails to grow progressively in nude mice and forms colonies only in enriched semisolid medium developed for tumour stem cells. The cells will grow in either low serum or serum free medium. In chick embryonic skin assay the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. LS 123 exhibits several properties associated with neoplasia, but have little invasive potential and may represent an early stage in tumour development.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 225
- In Vitro 1983;19:99 - PMID: 6826200
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.