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SPECIES: rat, Wistar-Commentry
TUMOR: carcinoma, hepatocellular
DSMZ catalog code: ACC 195
Morphology: epithelial-like cells growing as monolayers
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Description: rat hepatocarcinoma a primary liver carcinoma was induced by dimethyl-aminoazobenzene (DAB) in a Wistar-Commentry rat; the hepatoma was transferred to a syngeneic rat and a cell line was established and subcloned in 1966 confirmed as rat with IEF of AST, G6PD, MDH, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. C. Frayssinet, CNRS, Villejuif, France
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% Dulbecco's MEM + 5-10% FBS split confluent culture about every 2 days using trypsin/EDTA; cells grow quickly; seed about 1 x 106 cells into a 175 cm 2 flask at 37C with 5% CO2 cell harvest of about 30 x 106 cells/175 cm 2;
doubling time of ca. 15 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed as rat with IEF of AST, G6PDH, MDH, PEP B)
- Cancer Res 1978;38:16-22 - PMID: 201371
- Int J Cancer 1992;50:238-245 - PMID: 1309725
- Liver Cells and Drugs (A Guillouzo, John Libbey Eurotext ed) 1988;164:465-471
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.