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TISSUE/ORGAN: lymphoblastoid B cell
DSMZ catalog code: ACC 218
Morphology: lymphoblastoid cells growing singly and in large clumps in suspension
Description: human B-lymphoblastoid cells (from patient with melanoma) Epstein-Barr virus-positive cell line established from peripheral blood in 1977 from patient with melanoma confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% RPMI 1640 + 10-20% FBS maintain at about 0.3-1.0 x 106 cells/ml; optimal split ratio of 1:3 to 1:8 every 2-4 days at 37C with 5% CO2 saturation density at about 2.0 x 106 cells/ml;
doubling time of about 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 106 cells/ampoule;
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human near-tetraploid karyotype with diploid (36%) sideline and 4% polyploidy; 90(88-94)<4n>XX/XXY/XXYY; no consistent structural aberrations) * DNA fingerprinting (unique HinfI (gtg)5 DNA profile) * immunological analysis (CD3- CD10- CD13- CD19+ (50%) CD20+ (98%) CD37+ (80%) HLA-DR+ (98%) smIgG- (?) smIgM- smIgkappa- smIglambda cyIgG+ cyIgM- cyIgkappa+ cyIglamda-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.