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SPECIES: mouse, B10.BR
ECACC catalog code: 86060302
Description: Mouse B10.BR lymphoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Interleukin 2 (IL-2) Applications: IL-1 assay
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 15% Fetal Bovine Serum (FBS). Maintain cultures between 5x100,000 to 1,000,000 cells/ml; 5% CO2; 37C. Shake the flask to remove attached cells.
Derived from a lymphoma that occurred in a B10.BR mouse. The cells respond to Interleukin 1 (IL-1) stimulation by producing Interleukin 2 (IL-2) and can be used to assay IL-1 activity.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 8079
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.