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ECACC catalog code: 92040118
Description: Porcine peripheral blood B cell
Viruses: See Properties
Depositor: Dr B Kaeffer, INRA, Laboratoire Pathologie, Nouzilly, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner, somatic cell genetic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 5µg/ml 6-Thioguanine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: 2n = 40, diploid
L231 is derived from the parent line L23 and is HAT-sensitive. The cells contain retrovirus particles but are negative in RT assay.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Hybridoma 1991;10:731 - PMID: 1667778
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.