L23

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

L23 (pig, blood)

SPECIES: pig
TISSUE/ORGAN: blood

ECACC catalog code: 91012318
Morphology: Suspension
Description: Porcine peripheral blood B cell
Viruses: See Properties
Depositor: Dr B Kaeffer, INRA, Laboratoire Pathologie, Nouzilly, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin M (IgM) Applications: RT assay and virus studies: Porcine retrovirus

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37C. Cells grow as small clumps.
Passages: 67; Karyotype: Modal No 36.5
One of a series established from mononuclear cells of boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). The cells can contain retrovirus particles but are negative in RT assay.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Bibliographic references:

Int J Cancer 1990;46:481 - PMID: 2394515

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105