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ICLC catalog code: HTL97022
Continuous culture, grown as monolayer
SPECIES: human, Asiatic (male, 64 year) Karyology: aneuploid
TUMOR: carcinoma, squamous cell
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B have been reported. The cells express a large amount of epidermal growth factor receptors
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + Ham's F12 (1:1) + 2% FBS + 2 mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1x104 cells/cm2; 37C, 5% CO2. Passage: 340, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, MD,NP)
Availability in cell line catalogues: ECACC: 94072011, DSMZ: ACC 351
- Cancer 1992;69:277 - PMID: 1728357
- Int J Cancer 1994;58:291 - PMID: 7913084
- Int J Cancer 1996;65:372 - PMID: 8575860
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.