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SPECIES: human, Asiatic
TUMOR: carcinoma, squamous cell
ECACC catalog code: 94072011
Description: Human Asian squamous cell carcinoma
Viruses: See Properties
Depositor: Dr Y Shimada, First Department of Surgery, Faculty of Medicine, Kyoto University, Kyoto, JAPAN (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of human oesophageal cancer.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + Ham's F12 (1:1) + 2mM Glutamine + 2% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: HTL97022, DSMZ: ACC 351
- Cancer 1992;69:277 - PMID: 1728357
- Int J Cancer 1994;58:291 - PMID: 7913084
- Int J Cancer 1996;65:372 - PMID: 8575860
See homonymous cell lines in other collections and catalogues.
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.