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TISSUE/ORGAN: skin, fibroblast
ECACC catalog code: 90011873
Description: Human skin fibroblast, thioguanine resistant
Viruses: See Properties
Depositor: Dr C Arlett, MRC Cell Mutation Unit, University of Sussex, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Genetic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Primary fibroblast selected for resistance to 5µg/ml thioguanine.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.