KARPAS-299

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

KARPAS-299 (human, peripheral blood, lymphoma, T cell)

SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: lymphoma, T cell

DSMZ catalog code: ACC 31
Morphology: round to polygonal, growing singly or in small clusters in suspension
Description: human T cell lymphoma established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin lymphoma in 1986; now classified as 'CD30+ anaplastic large cell lymphoma' (ALCL); cells carry the NPM-ALK fusion gene confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. A. Karpas, University of Cambridge, Cambridge, UK
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 1.0-2.0 x 106 cells/ml; split 1:3 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximal density of about 2-3 x 106 cells/ml; doubling time of ca. 30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypodiploid karyotype with 14% polyploidy; 44(42-45)<2n>XY, -10, -22, t(1;17)(q22;p11), t(2;5)(p23;q35), del(6)(q23), der(13)t(13;?)(p12;?), der(14)t(14;22)(p12;q11), der(19)t(19;?)(q13;?), del(22)(q12)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2- CD3- CD4+ CD5+ CD6- CD7+ CD8- CD13+ CD19-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (not detected (RDDP assay), negative for HTLV-I/II, HIV-1)

Bibliographic references:

Blood 1988;72:234-240 - PMID: 3260522

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105