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ECACC catalog code: 88102402
Susceptibility to viruses: poliovirus 1
Description: Human amnion, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: poliovirus 1
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-5x100,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 46
This cell line was derived from normal human amnion and shown to be contaminated with HeLa cells. They are mainly used for virus studies (susceptible to poliovirus 1). HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (Species confirmation. G6PD type A)
Availability in cell line catalogues: ATCC: CCL 21
- Nature 1976;259:211-213 - PMID: 1250349
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.