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ECACC catalog code: 92120308
Description: Human choriocarcinoma
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Properties: Products: Human chorionic gonadotrophin (hCG), somatomammotrophin
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin, 5% CO2; 37C.
Karyotype: 2n = 46, modal No 71
One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 36
- J Clin Endocrinol 1971;32:683-687 - PMID: 5103722
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.