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TUMOR: leukemia, T cell
ECACC catalog code: 96060401
Description: Human T cell, mutant derivative of Jurkat
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of apoptosis, signal transduction receptor analysis.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
The T cell leukaemia line J.CaM1.6 was derived from the parent cell line Jurkat E.6-1 (ECACC No. 88042803). Jurkat cells were mutagenised with ethyl methanesulfonate and subsequently grown in phytohemagglutinin containing media for 2 weeks. J.CaM1.6 express a structurally Normal but functionally defective CD3/Ti complex. The cells fail to exhibit inositolphospholipid metabolism or Ca2+ mobilisation in response to anti-CD3 or anti-Ti monoclonal antibodies. They display resistance to anti-CD3 induced apoptosis, constitutively express Fas and are sensitive to anti-Fas induced apoptosis. It was shown that J.CaM1.6 cells are defective in early signalling events that follow TCR stimulation owing to loss of lck tyrosine kinase function due to the lack of exon 7 in the lck transcript.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2063
- Biochem Biophys Res Commun 1995;213:994 - PMID: 7544583
- Cell 1992;70:585 - PMID: 1505025
- Proc Natl Acad Sci U S A 1987;84:6879 - PMID: 3309950
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.