IPL-LD-65Y

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

IPL-LD-65Y (Lymantria dispar (gypsy moth), larvae)

SPECIES: Lymantria dispar (gypsy moth)
TISSUE/ORGAN: larvae

DSMZ catalog code: ACC 181
Morphology: large cells; up to 30% grow adherent with processes; suspension cells are round to oval
Description: insect - gypsy moth (Lymantria dispar) established from the larvae of a gypsy moth (species Lymantria dispar, family Lymantriidae, order Lepidoptera) (Schwammspinner) confirmed as not murine and not primate with IEF of AST, MDH, PEP B
Depositor: Dr. H. S. Conradt, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% TC 100 medium + 20% FBS cells are difficult to culture and grow slowly! split confluent culture 1:4 every 4-5 days using a (plastic) cell scraper without exposure to CO2 (keep lid of flask closed) at room temperature (20-25C) cell harvest of up to 10 x 106 cells/80 cm 2; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed as not murine and not primate with IEF of AST, MDH, PEP B)

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105