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DSMZ catalog code: ACC 251
Morphology: adherent, spindle-like cells (a few epitheliod cells) growing as confluent monolayer
Description: human melanoma established from the primary tumor (right cervical) of a 64-year-old woman with cutaneous melanoma confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. C. Aubert, Institut Paoli Calmette, Marseille, France
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:8 to 1:10 every 4-5 days using trypsin/EDTA; seed out at ca. 3 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 0.1 x 106 cells/cm 2;
doubling time of about 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human flat-moded hypertriploid karyotype with 13% polyploidy; 74(74-88)<3n>XX, -X, +1, -2, +3, -4, +6, +7, +9, -11, -11, +12, -13, -14, - 15, +16, +21, +21, +4mar, dmin in 34% cells, del(3)(p14)x2, del(3)(q26.3)x2, del(6)(p23),) * cytogenetics 2 (del(7)(p14), add(7)(p22;HSR), der(9)t(9;11)(p21;q11), add(12)(p13)x3, del(12)(p13), del(17)(p12)x1-2; sdls with additional numerical and structural ch7 rearrangements) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin-, desmin-, endothel-, GFAP-, neurofilament+ (40%), vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- Int J Cancer 1993;54:784-792 - PMID: 8325707
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.