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SPECIES: human, Caucasian
TISSUE/ORGAN: skin, fibroblast
PATHOLOGY: methylmalonicacidemia*251000 (OMIM record)
ECACC catalog code: 90112605
Susceptibility to viruses: herpes simplex * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
Description: Human Caucasian skin methylmalonicacidemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Genetic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 2% Amino Acids + 2% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 46
Derived from the skin biopsy of a one year-old Caucasian male with methylmalonicacidemia. The cells have a limited lifespan; up to 40 passages are expected.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 124, IZSBS: BS CL2
- Pediatr Res 1969;3:217 - PMID: 5788784
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.