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DSMZ catalog code: ACC 239
Morphology: adherent epithelial-like cells growing in monolayers
Description: human melanoma established from the primary (achromic) cutaneous tumor (left thigh) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); same patient as cell line IGR-37 (DSM ACC 237); described as tumorigenic in nude mice confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. C. Aubert, Institut Paoli Calmettes, Marseille, France
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% Dulbecco's MEM + 15% FBS split subconfluent or confluent culture 1:4 every 3-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 ; optimal density of about 4 x 106 /175 cm 2 at 37C with 5% CO2 maximal density of about 8 x 106 /175 cm 2;
doubling time of about 18 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (apparently human karyotype) * DNA fingerprinting (identical with IGR-37, but otherwise unique DNA profile with (gtg)5 multilocus and pYNH24 single locus probes) * immunological analysis (cytokeratin-, desmin-, endothel-, GFAP-, neurofilament-, vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
- J Natl Cancer Inst 1980;64:1029-1040 - PMID: 6929009
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.