HTC

Version 4.0.201701

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

HTC (rat, buffalo, ascitic fluid, hepatoma)

Monoclonal continuous culture, grown as suspension
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
tumorigenic in syngeneic animals
Cell line available under some conditions.
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor

Available in the following LABORATORY:

Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-30, mycoplasma negative, HOECHST
Growth also as monolayer. Derived from hepatoma induced by N,N
Validated by: beuraldehyde dehydrogenase (high levels) * chromosome analysis (large metacentric chromosome 1)

Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

See homonymous cell lines in other collections and catalogues.

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105