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ECACC catalog code: 95091411
Transfected by: Transfection Back To Transfection WebPage
Description: Human cervix carcinoma, HeLa derivative, transformed
Viruses: See Properties
Depositor: Prof H Bujard, ZMBH, Heidelberg, GERMANY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Gene expression and activation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
HR5-CL11 cells were obtained after transfection of HeLa cells with plasmids coding for a transcriptional transactivator and transferring G418 and hygromycin resistance. This tetracycline-controlled transactivator (a fusion between the tet repressor and the activating domain of the herpes simplex virus VP16 transcription activation domain) can stimulate the expression of a reporter gene up to 100,000 fold. Subsequently these cells were stably transfected with a luciferase reporter unit. Luciferase expression in the HR5-CL11 cells is highly stimulated by doxycycline.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Science 1995;268:1766 - PMID: 7792603
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.