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SPECIES: human, Caucasian
TISSUE/ORGAN: foreskin, fetal
ECACC catalog code: 86031405
Susceptibility to viruses: cytomegalovirus * herpes simplex
Description: Human Caucasian fetal foreskin fibroblast
Viruses: See Properties
Depositor: Dr G Farrar, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: CMV and HSV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from a 14-18 week human foetus. The cells support growth of CMV and HSV. This cell line has a finite life span.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: HL95002
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.