This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
SPECIES: mouse / human
ECACC catalog code: 90012609
Description: Human (WI-L2-729-FF2) x mouse (P3X63/Ag8.653) heterohybridoma
Viruses: See Properties
Depositor: Dr S Clark, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Parent: WI-L2-729-HF2. Murine myeloma: P3X63/Ag8.653. Generated by electro-acoustic fusion of 2 lines. Non-immunoglobulin secreting, HAT-sensitive, resistant to ouabain at greater than 100uM, will fuse with normal human lymphocytes and lymphoblastoids.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.