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ICLC catalog code: HTL97015
Continuous culture, grown as monolayer
polygonal flat to spindle-like morphology
SPECIES: human (male, 42 years old) Karyology: complex chromosomal changes; p53 mutation
TUMOR: carcinoma, follicular
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cell line has been used for studies on the function of human thyroid and development of thyroid cancer; expression of 5-deiodinase type I; the cells retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors; thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-5x104 cells/cm2. 37C, 5% CO2. Passage: 12, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with G6PD, NP.)
Availability in cell line catalogues: ECACC: 94060901
- Front Horm Res 1989;18:56
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.