F4/4.K6

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

F4/4.K6 (mouse, (C57BL/10xC57BL/10.BR), lymphocyte, T)

SPECIES: mouse, (C57BL/10xC57BL/10.BR)
TISSUE/ORGAN: lymphocyte, T

DSMZ catalog code: ACC 52
Morphology: single or clustered cells in suspension
Description: mouse T cells established from a bovine insulin-specific CD4+ T cell line (C57Bl/10 x C57Bl/10.BR H-2 b x H-2 k ); cell line can be used as assay for IL-4
Depositor: Dr. E. Schmitt, Institute of Immunology, Mainz, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
CG medium + 50 U/ml mouse IL-4 (strictly without FBS) maintain at 0.2-0.4 x 10 6 cells/ml; split 1:2; upon thawing of frozen cells, cells must be washed thoroughly as already tiny amounts of FBS willl lead to adherence at 37C with 5% CO2 saturation density at about 0.5 x 106 cells/ml; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture assays;

Bibliographic references:

Eur J Immunol 1990;20:1709-1715 - PMID: 2120063

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105