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SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
TUMOR: leukemia, chronic myeloid
DSMZ catalog code: ACC 135
Morphology: single, round to oval cells in suspension
Description: human chronic myeloid leukemia in blast crisis established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia-chromosome positive chronic myeloid leukemia (CML) in second relapse in 1980 (after bone marrow transplantation day +28); sister cell line of EM-3 (DSM ACC 134) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. A. Keating, Toronto General Hospital, Ontario, Canada
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-1.0 x 106 cells/ml; split ratio of 1:2 at 37C with 5% CO2 maximum density at about 1.0-1.5 x 106 cells/ml;
doubling of ca. 35-40 h;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypertriploid karyotype with hypotetraploid sideline; 74(70-86)<3n>X,-X,-X, +3, +4, +6, +6, +6, +8, -9, +11, -14, -14, +15, +17, -19, +21, +22, +mar, der(5)t(5;?)(q13/15;?), der(9)t(9;22)(q34;q11), i(17q)x2; in some cells up to tree) * cytogenetics 2 (copies of der(9) are present; the karyotype overlaps that of EM-3 (DSM ACC 134) and corresponds with those reported for both EM-2 and EM-3) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile, but similar to EM-3) * immunological analysis (CD3+ CD13+ CD14+ CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
- Cancer Genet Cytogenet 1987;25:271-284 - PMID: 3030532
- Clin Haematol 1987;1:1021-1029
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.