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SPECIES: human, Caucasian
DSMZ catalog code: ACC 231
Morphology: epitheloid, growing in clusters at first, then as monolayer
Description: human breast carcinoma established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979; cells were received at passage 53, frozen at DSMZ at passage 56 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. Dr. F. Hoelzel, Women's University Hospital Eppendorf, Hamburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA; seed out at ca. 2 x 106 cells/25 cm 2 at 37C with 5% CO2 cell harvest of about 1.5-2.0 x 106 cells/cm 2;
doubling time of about 64 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human highly rearranged hypotriploid karyotype with 5.8% polyploidy; 62(58-65)<3n>X, der(X), apparent ch17 nullisomy and preponderance of marker chromosomes, e.g. add(1)(p11), i del(1)(q11q?22), i(1p), i(2p), add(4)(p11), add(4)( 11), e) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- In Vitro 1984;20:157-166 - PMID: 6325325
- J Clin Endocinol Metabol 1985;60:1243-1249 - PMID: 2987289
- J Clin Endocrinol Metab;60
- J Natl Cancer Inst 1984;73:313-321 - PMID: 6589426
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.