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TISSUE/ORGAN: trachea, embryonic
ECACC catalog code: 87090202
Susceptibility to viruses: bovine diarrhea virus * herpes simplex * infectious bovine rhinotracheitis * parainfluenza 3 * reovirus 3 * swine fever virus * vesicular stomatitis (Ogden strain)
Description: Bovine fetal trachea
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: BVDV, IBRV and parainfluenza type 3 virus.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 60
Derived from the trachea of a male bovine foetus. The cell line can be used for isolation, replication and quantitation of BVDV, IBRV and parainfluenza type 3 virus. Growth and morphological characteristics decline beyond passage number 55.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 44, IZSBS: BS CL 39
- 16° Ann Meeting TCA Abstract 1965
- Am J Vet Res 1968;29:1003 - PMID: 4967124
- Excerpta Medica 1965;19(sect 1)
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.