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TISSUE/ORGAN: B cell
PATHOLOGY: chronic Hepatitis C (OMIM
ECACC catalog code: 94111519
Description: Human IgG1 secreting B cell
Viruses: See Properties
Depositor: Prof M Mondelli, Dr A Cerino, Instituto di Clinica delle Malatte Infettive, IRCCS Policlinico San Matteo, Pavia, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG1) (kappa) specific for Hepatitis C virus NS4 region Applications: Used in HCV typing studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 20u/ml IL-6 + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x10,000 cells/ml; using 0.25% trypsin or trypsin EDTA; 5% CO2: 37C.
Derived from a patient with chronic Hepatitis C (HCV type 1). Antibody specific to non structural protein 4a (NS4a) of Hepatitis C virus. Fine specificity mapped to amino acid residues 1697-1708 of HCV deduced amino acid sequence. The cells should be handled under laboratory containment level 2 conditions.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Immunol 1991;147:2692 - PMID: 1717573
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.