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SPECIES: human, Caucasian
TISSUE/ORGAN: skin, fibroblast
PATHOLOGY: trisomy 21 / Down syndrome#190685 (OMIM record)
ECACC catalog code: 87021203
Susceptibility to viruses: poliovirus 1
Description: Human, Down syndrome
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% Luteinising Hormone (LH) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 6% CO2; 37 C. Finite lifespan up to 30 passages expected.
Karyotype: 2n = 46, aneuploid
Derived from a 2 and a half year old year old caucasian female who exhibited symptoms of Down Syndrome.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 66, IZSBS: BS CL29
- Proc Southern Soc Ped Res 1964;5:12
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.