JCRB0624 HSC-4

Cell information

Cell type：general cells (Charges for cells)

JCRB No.   JCRB0624 Cell Name   HSC-4
profile   Human squamous carcinoma cell line from tongue. Alias   
animal   human strain   
genus   Homo species   sapiens
sex   M age   64
identity   available tissue   oral
case history   cancer in tongue. metastasis   
metastorgn    genetics   squamous carcinoma, tongue, age 64, (M)
lifespan   infinite crisispdl   
morphology   epithelial-like charact   squamous carcinoma
classify   tumor originator   Momose,F.
register   Momose,F. regulation   no
comment    dyear   
medium   Eagle's minimal essential medium with 10% calf serum. pasmethod   Cells are treated with trypsin and EDTA. Cells are diluted 1/8 once a week for the subculture.
pascellno   1 x 10^5 cells/10cm dish race   Japanese
co2conc   5 % tohroku   registered
preporgn   tongue vrsummary   tested
vrsdetect   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] tissueform   


References
Pubmed id:2164440 Potent growth inhibition of human tumor cells in culture by arginine deiminase purified from a culture medium of a Mycoplasma-infected cell line.
Miyazaki K,Takaku H,Umeda M,Fujita T,Huang WD,Kimura T,Yamashita J,Horio T
Cancer Res. 1990 Aug 1;50(15):4522-7
Pubmed id:2228902 Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium.
Rikimaru K,Toda H,Tachikawa N,Kamata N,Enomoto S
In Vitro Cell Dev Biol. 1990 Sep;26(9):849-56
Images



LOT Information

Cell No.   JCRB0624 Cell Name   
LOT No.   06292012 LOTSPEC   distribution
MEDIUM   Eagle's minimum essential medium with 10% newborn calf serum (GIBCO Cat. # 16010) GROWTEMP   37 C
PASCELLNO   2.5 - 6.3 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
GROWTHRATE   approx. 25 hrs FREEZCONC   1.6 x 10^6
VIABLFREEZ   97.6 ANTIBIOTIC   free
PASSAGE   Unknown (8 at bank) PDL   
MYCO   - BACT   -
PROTOZOA    ISOZYME   Confirmed as human by NP, G6PD (type B), MD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% CS - EMEM CO2   5%
SCFREQ    RFLP   
FROMLOT   


Cell No.   JCRB0624 Cell Name   
LOT No.   04012002 LOTSPEC   distribution
MEDIUM   Eagle's minimal essential medium with 10% fetal bovine serum GROWTEMP   37 C
PASCELLNO   4-6 x 10^4 cells/ml PASMETHOD   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
GROWTHRATE   D.T. = ca 25 hrs FREEZCONC   1.5 x 10^6
VIABLFREEZ   92 ANTIBIOTIC   free
PASSAGE   P9* PDL   
MYCO   - BACT   -
PROTOZOA    ISOZYME   Confirmed as human by NP, G6PD (type B), MD, LD.
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD    EXOGENE   
FMEDIUM   10% DMSO, 20% FBS-EMEM CO2   5%
SCFREQ    RFLP   
FROMLOT   


Cell No.   JCRB0624 Cell Name   HSC-4
LOT No.   112696 LOTSPEC   Distribution
MEDIUM   Eagle's minimal essential medium with 10% calf serum (C.C.Labs.10910-01). GROWTEMP   37 C
PASCELLNO   1-1.5x10^5 cells/ml PASMETHOD   Wash cells with PBS(-) and treat them with 0.02% EDTA and 0.25% trypsin for 10 min at 37 C.
GROWTHRATE   NT FREEZCONC   1.2x10^6
VIABLFREEZ   95.0 ANTIBIOTIC   free
PASSAGE   P4* PDL   NT
MYCO   - BACT   -
PROTOZOA   NT ISOZYME   G6PD(type B), NP, LDH examined. (human).
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM   Culture medium with 5% DMSO. CO2   5%
SCFREQ    RFLP   
FROMLOT   950823


Images
Cell No.   JCRB0624 Cell Name   HSC-4
LOT No.   091985 LOTSPEC   distribution
MEDIUM   MEM + 10%CS GROWTEMP   37
PASCELLNO   1 X 10^5 PASMETHOD   trypsin-EDTA, 1:8 dilution once a week
GROWTHRATE   NT FREEZCONC   
VIABLFREEZ   NT ANTIBIOTIC   
PASSAGE    PDL   
MYCO   - BACT   -
PROTOZOA    ISOZYME   NT
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD    EXOGENE   
FMEDIUM    CO2   
SCFREQ    RFLP   
FROMLOT   


Cell No.   JCRB0624 Cell Name   
LOT No.   940606 LOTSPEC   distribution
MEDIUM   MEM + 10%CS GROWTEMP   37
PASCELLNO    PASMETHOD   trypsin-EDTA, 1:8 dilution once a week
GROWTHRATE    FREEZCONC   
VIABLFREEZ    ANTIBIOTIC   kanamycin 60 ug/ml
PASSAGE    PDL   
MYCO    BACT   
PROTOZOA    ISOZYME   
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD    EXOGENE   
FMEDIUM    CO2   
SCFREQ    RFLP   
FROMLOT   


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