IFO50414 CHO-K1
Cell information
Cell type：general cells (Charges for cells)
| JCRB No. | IFO50414 | Cell Name | CHO-K1 |
|---|---|---|---|
| profile | CHO-K1 cells are useful for isolation of nutritionally deficient mutants. | Alias | |
| animal | hamster, Chinese | strain | Chinese |
| genus | Cricetulus | species | griseus |
| sex | age | ||
| identity | tissue | ovary | |
| case history | metastasis | ||
| metastorgn | genetics | ||
| lifespan | infinite | crisispdl | |
| morphology | epitherial like | charact | widely used for production of recombinant proteins |
| classify | originator | Puck, T. T. | |
| register | Takeuchi,M. | regulation | |
| comment | dyear | 1993 | |
| medium | Ham's F12 medium with 10% fetal bovine serum | pasmethod | trypsin-EDTA |
| pascellno | race | ||
| co2conc | 5 % | tohroku | registered |
| preporgn | ovary | vrsummary | |
| vrsdetect | tissueform |
LOT Information
| Cell No. | IFO50414 | Cell Name | |
|---|---|---|---|
| LOT No. | 07072008 | LOTSPEC | distribution |
| MEDIUM | Ham's F12 medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) | GROWTEMP | 37 C |
| PASCELLNO | inoculum 10^5 cells/ml -> confluent after 2 days. | PASMETHOD | 0.25% trypsin and 0.02% EDTA |
| GROWTHRATE | 17 hrs | FREEZCONC | 3.0 x 10^6 |
| VIABLFREEZ | 98.7 | ANTIBIOTIC | free |
| PASSAGE | Unknown (8 at bank) | PDL | |
| MYCO | - | BACT | - |
| PROTOZOA | ISOZYME | Confirmed as Chinese hamster by NP, G6PD, MD. | |
| CHRMODE | CHRDISTRB | ||
| SURFACEAG | DNA Profile (STR) | ||
| ANCHORDEPD | Yes | EXOGENE | |
| FMEDIUM | 10% DMSO, 20% FBS - F12 | CO2 | 5% |
| SCFREQ | RFLP | ||
| FROMLOT |
| Cell No. | IFO50414 | Cell Name | |
|---|---|---|---|
| LOT No. | 06022003 | LOTSPEC | distribution |
| MEDIUM | Ham's F12 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | GROWTEMP | 37 C |
| PASCELLNO | 5 x 10^4 cells/ml | PASMETHOD | |
| GROWTHRATE | D.T. = 13 - 23 hrs | FREEZCONC | 3.2 x 10^6 |
| VIABLFREEZ | 97 | ANTIBIOTIC | free |
| PASSAGE | Unknown(7 at bank) | PDL | |
| MYCO | - | BACT | - |
| PROTOZOA | ISOZYME | Confirmed as Chinese hamster by NP, G6PD, AST. | |
| CHRMODE | CHRDISTRB | ||
| SURFACEAG | DNA Profile (STR) | ||
| ANCHORDEPD | Yes | EXOGENE | |
| FMEDIUM | 10% DMSO, 20% FBS-Ham's F12 | CO2 | 5% |
| SCFREQ | RFLP | ||
| FROMLOT |
| Cell No. | IFO50414 | Cell Name | CHO-K1 |
|---|---|---|---|
| LOT No. | 1132 | LOTSPEC | distribution |
| MEDIUM | Ham's F12, 90% ; FBS, 10% | GROWTEMP | 37 C |
| PASCELLNO | 5-10 x 10^3 cells/ml | PASMETHOD | trypsin-EDTA |
| GROWTHRATE | FREEZCONC | 1.8 x 10^6 | |
| VIABLFREEZ | 89.0 | ANTIBIOTIC | free |
| PASSAGE | Unknown (5 at IFO) | PDL | |
| MYCO | - | BACT | - |
| PROTOZOA | NT | ISOZYME | NP, G6PD, PepB |
| CHRMODE | CHRDISTRB | ||
| SURFACEAG | DNA Profile (STR) | ||
| ANCHORDEPD | EXOGENE | ||
| FMEDIUM | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 | |
| SCFREQ | RFLP | ||
| FROMLOT |