IFO50380 TLR3
Cell information
Important Notice(s)Cell type：general cells (Charges for cells)
| JCRB No. | IFO50380 | Cell Name | TLR3 |
|---|---|---|---|
| profile | P450IA1 mRNA is induced by methylcholanthrene. | Alias | |
| animal | mouse | strain | C57BL/6 transgenic |
| genus | Mus | species | musclus |
| sex | age | ||
| identity | tissue | liver | |
| case history | hepatocyte | metastasis | |
| metastorgn | genetics | TG mouse harboring tsSV40 large T antigen gene | |
| lifespan | infinite | crisispdl | |
| morphology | charact | hepatocyte immortalized by the tsSV40 large T antigen gene. Permissive temperature is 33 C. | |
| classify | transformed | originator | Yanai, N. et al. |
| register | Yanai,N. | regulation | |
| comment | dyear | 1992 | |
| medium | RITC80-7 with 2% fetal calf serum, 10ng/ml EGF, 5ug/L monoethanolamine,10ug/ml Tf & 1ug/ml insulin | pasmethod | 25 ug/ml pronase-Hanks'BSS, collagen-coated substratum is required |
| pascellno | race | ||
| co2conc | 5 % | tohroku | registered |
| preporgn | liver | vrsummary | |
| vrsdetect | tissueform |
LOT Information
| Cell No. | IFO50380 | Cell Name | |
|---|---|---|---|
| LOT No. | 06072002 | LOTSPEC | distribution |
| MEDIUM | RITC80-7 with 2% FCS, 10ng/ml EGF, 5ug/L monoethanolamine, 10ug/ml transferrin, 1ug/ml insulin | GROWTEMP | 33 C. This cell line does not grow at 37 C. |
| PASCELLNO | 1.5-2.0 x 10^5 cells/ml | PASMETHOD | 0.25% trypsin and 0.02% EDTA. Collagen-coat is needed. |
| GROWTHRATE | FREEZCONC | 4.1 x 10^6 | |
| VIABLFREEZ | 91.2 | ANTIBIOTIC | free |
| PASSAGE | P14 | PDL | |
| MYCO | - | BACT | - |
| PROTOZOA | ISOZYME | Confirmed as mouse by NP. MD, AST. | |
| CHRMODE | CHRDISTRB | ||
| SURFACEAG | DNA Profile (STR) | ||
| ANCHORDEPD | Yes | EXOGENE | |
| FMEDIUM | 10% DMSO, 20% FCS-culture medium | CO2 | 5% |
| SCFREQ | RFLP | ||
| FROMLOT |
| Cell No. | IFO50380 | Cell Name | TLR3 |
|---|---|---|---|
| LOT No. | 859 | LOTSPEC | seed/distribution |
| MEDIUM | RITC80-7 with 2%FCS, 10ng/ml EGF, 5 ug/L monoethanolamine, 10 ug/ml Tf, 1 ug/ml insulin | GROWTEMP | 33 C |
| PASCELLNO | PASMETHOD | 25 ug/ml pronase-Hanks'BSS ; collagen-coated culture dish | |
| GROWTHRATE | FREEZCONC | 4 x 10^6 | |
| VIABLFREEZ | 91.0 | ANTIBIOTIC | free |
| PASSAGE | P11 | PDL | |
| MYCO | - | BACT | - |
| PROTOZOA | NT | ISOZYME | NP, G6PD, MD |
| CHRMODE | CHRDISTRB | ||
| SURFACEAG | DNA Profile (STR) | ||
| ANCHORDEPD | EXOGENE | ||
| FMEDIUM | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 | |
| SCFREQ | RFLP | ||
| FROMLOT |