Abstract: The nucleosome core particle is the fundamental repeating subunit of chromatin. It consists of two molecules each of the four ‘core histone ' proteins, H2A, H2B, H3 and H4, and a 147 bp stretch of DNA. A better knowledge of the chromatin nucleosomal organization is crucial to understand many important phenomena occurring in chromosomes. Regulatory mechanisms of gene expression are partially influenced by nucleosome positioning and regions with exposed chromatin (i.e. where nucleosomes are more distant) can be more prone than others to double strand breaks. Analysis of nucleosomal DNA has demonstrated the existence of a weak sequence-dependent signal for nucleosome positioning, this makes classical computational biology methods, like alignment and consensus sequences, poorly applicable here. The ability of DNA to assume certain conformation in certain positions can considerably enhance its binding potential to nucleosomes. According to recent X-ray structure studies, the 147 bp nucleosomal DNA has detectable bends symmetrically displaced around the central position, this suggests the presence of localized periodicities in DNA bendability. Wavelet transform can be used to locally evaluate periodicities allowing to detect positions with a bend distribution similar to known nucleosomal DNA.