Abstract: Splice site consensus values (CVs) are usually calculated using previously described matrices [1] which are obtained through the analysis of a relatively small splice site number (1500) from different organisms. Now, genome annotation becoming complete, a much more accurate definition is possible. Furthermore, recent studies [2, 3, 4] indicate that consensus value itself is not sufficient to define splice site strength and other parameters must be considered to improve splice site definition. To investigate how intron and exon lengths might be exploited by the splicing machinery to ensure proper splicing control and regulation a human intron database has been developed and analyzed.