1. Barberis M, De Gioia L, Ruzzene M, Sarno S, Coccetti P, Pinna LA, Vanoni M, Alberghina L
The Cyclin-Dependent Kinase Inhibitor Sic1 of Saccharomyces cerevisiae Is a Functional and Structural Homologous to the Mammalian p27Kip1
Meeting: BITS 2004 - Year: 2004
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Abstract: In budding yeast Sic1, an inhibitor of cyclin-dependent kinase (Cki), blocks the activity of Cdk1-Clb5/6 (S-Cdk1) kinase required for the initiation of DNA replication that takes place only when Sic1 is removed . Deletion of Sic1 causes premature DNA replication from fewer origins, extension of the S-phase and inefficient separation of sister chromatids during anaphase, whereas delaying S-Cdk1 activation rescues both S and M phase defects. Despite the well documented relevance of Sic1 inhibition on S-Cdk1 for cell cycle control and genome instability, the mechanism by which Sic1 inhibits S-Cdk1 activity remains obscure. Sic1 has been proposed to be a functional homologous of mammalian Cki p21Cip1, that is characterized by a significant sequence similarity with Cki p27Kip1, inhibitor of the Cdk2/Cyclin A kinase activity during S-phase.