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For information, get in touch with: Dott.ssa Barbara Parodi
Email: barbara.parodi@hsanmartino.it
  P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105


Sp2/0-Ag14 (mouse, BALB/c, hybrid, myeloma)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: hybrid
TUMOR: myeloma
Original line: P3X63Ag8 x BALB/c mouse spleen cell
Transformed by: fusion with anti SRBC spleen cells by PEG
tumorigenic in syngeneic animals
PROPERTIES: Ig non synthesized; resistant to 8-azaguanine; fusion partner; monoclonal antibody prod.

Available in the following LABORATORY:

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401, DSMZ: ACC 146

Bibliographic references:


Sp2/0-Ag14 (mouse / mouse, myeloma)


SPECIES: mouse / mouse
TUMOR: myeloma

DSMZ catalog code: ACC 146
Morphology: single, round cells in suspension
Transformed by: Mutations Back To Mutations WebPage
Description: mouse myeloma SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 ęg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies confirmed as mouse with IEF of LDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. G. Koehler, Max-Planck-Institute, Freiburg, Germany
General Restrictions

Available in the following LABORATORY:

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401

Bibliographic references:


Sp2/0-Ag14 (mouse, BALB/c, hybrid, myeloma)


SPECIES: mouse, BALB/c
TISSUE/ORGAN: hybrid
TUMOR: myeloma

ECACC catalog code: 85072401
Morphology: Suspension
Transformed by: Mutations Back To Mutations WebPage
Description: Mouse x Mouse hybridoma, non-producing
Viruses: See Properties
Depositor: Dr P Wilton-Smith, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner

Available in the following LABORATORY:

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, DSMZ: ACC 146

Bibliographic references:


Sp2/0-Ag14 (mouse, BALB/c, hybrid, myeloma)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: hybrid
TUMOR: myeloma
Original line: P3X63Ag8 x BALB/c mouse spleen cell
Transformed by: fusion with anti SRBC spleen cells by PEG
tumorigenic in syngeneic animals
PROPERTIES: Ig non synthesized; resistant to 8-azaguanine; fusion partner; monoclonal antibody prod.

Available in the following LABORATORY:

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401, DSMZ: ACC 146

Bibliographic references:



For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
Email: barbara.parodi@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105