TISSUE/ORGAN: bone marrow
ECACC catalog code: 94092304
Morphology: Epitheloid and aggregate-forming populations
Description: Human neuroblastoma
Viruses: See Properties
Depositor: Dr C Helson, St Agnes Hospital, New York, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Transferrin receptor Applications: Carcinogenesis studies, cytotoxicity assays.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Clumped cells have tendency to float.
SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: HTL97020
- Anticancer Drugs 1993;4:487 - PMID: 8104544
- J Natl Cancer Inst 1984;73(1):51 - PMID: 6610792
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.