SPECIES: fish - trout, rainbow
ECACC catalog code: 90102529
Susceptibility to viruses: arbovirus * eastern equine encephalitis * Egtved virus * infectious pancreatic necrosis * Venezuelan equine encephalitis
Description: Trout Rainbow gonad tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Passage using 0.25% trypsin below 24C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 4-24C.
Karyotype: 2n = 60
From pooled male/female gonad tissue of yearling rainbow trout. First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 55, DSMZ: ACC 352
- Proc Soc Exp Biol Med 1964;116:190
- Science 1962;135:1065
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.