SPECIES: human, Caucasian
TUMOR: leukemia, B-prolymphocytic
ECACC catalog code: 96090516
TRANSFORMED BY: Epstein-Barr
Description: Human Caucasian B-prolymphocytic leukaemia, EBV transformed
Viruses: See Properties
Depositor: Dr J V Melo, Department of Haematology, RPMS, Hammersmith Hospital, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of B cell leukaemia
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 4mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
JVM-13 has been derived from peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 express surface-membrane and cytoplasmic lambda light chain immunoglobulin (Ig). Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.
Research council deposit: No;
Price Code: C;
Availability in cell line catalogues: DSMZ: ACC 19
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.