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SPECIES: mouse, AKR/J(H-2k)
TISSUE/ORGAN: lymphocyte, helper T
DSMZ catalog code: ACC 45
Morphology: single, round cells in suspension
Description: mouse T-helper cells clone established from an AKR/J(H-2 k ) mouse; indicator cell line for IL-1 confirmed as mouse with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. Quentmeier, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
propagation medium: 90% RPMI 1640 + 10% FBS + 2 mM L-glutamine + 30 U/ml rh or rm IL-2 (CTLL units) maintain at 5-30 x 104 cells/ml; split ratio 1:3 to 1:4 every 3 days; cells have to be feedered every two weeks with spleen cells of H-2 k mice; feeder medium: 90% RPMI 1640 + 10% FBS + 2 mM L-glutamine + 10% (vol) supernatant of Con A-stimulated mouse spleen cells, 0.05 M methyl-alpha-D-mannopyranoside, 200 ęg/ml conalbumin; seed out at ca. 3 x 105 cells/ml at 37C with 7.5% CO2 maximal density at 0.6-1.0 x 106 cells/ml;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5-2.0 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: immunological analysis (half maximal proliferation with about 10pg rh IL-1a/ml in MTT assay)
Availability in cell line catalogues: ATCC: TIB 224
- J Exp Med 1983;158:836 - PMID: 6193236
- J Immunol 1984;133:2291-2293 - PMID: 6237148
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.