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SPECIES: mouse, Swiss albino
ECACC catalog code: 86120801
Susceptibility to viruses: herpes simplex * pseudorabies * vaccinia * vesic. stomatitis (Indiana)
Description: Mouse Swiss Albino embryo fibroblast
Viruses: See Properties
Depositor: Dr E I Levene, Department of Pathology, University of Cambridge, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Collagen and hyaluronic acid
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:20 i.e. seeding at 5x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 40
Derived from Swiss mouse embryo's this cell line secretes collagen and hyaluronic acid. Unlike 3T3, the 3T6 line is not contact inhibited.
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 96
- J Cell Biol 1963;17:299-313
- Virology 1965;27:179-185 - PMID: 4284655
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.