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ICLC catalog code: ATL06001
Continuous culture, grown as monolayer
SPECIES: mouse, 129/SV (male)
DEPOSITOR: Dr S. Astigiano, Transgenic Animals Dept, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: pluripotent; differentiation
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM (with 4.5 g/l glucose) + 10% Calf serum; Freezing medium: 95% FCS + 5% DMSO; split 1:5 - 1:10, 3 times per week; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Growth on gelatin-coated dishes. Differentiation into visceral or parietal endoderm with retinoic acid or HMBA treatment
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers)
- Cell 1975 ;5(3):229-43 - PMID: 1097123
- Differentiation 1984;27(3):236-42 - PMID: 6500205
- Exp Cell Res 1979;124(2):381-91 - PMID: 510422
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.