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ICLC catalog code: HTL04001
Continuous culture, grown as monolayer
TISSUE/ORGAN: kidney, embryonic
Original line: 293
TRANSFORMED BY: SV40
DEPOSITOR: Dr R. Notaro, Human Genetics Dept IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: SV40 large T antigen expression. Used for transfection assays and as packaging cells. The cell line can be transiently transfected and give extremely high levels of expression of AP fusion proteins. G418 resistant, neomicine resistant. The cell line is known also as 293tsA1609neo.
Available in the following LABORATORY:
IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; Split subconfluent cultures (70% confluence)1:4-1:8 using trypsin/EDTA; 37°C, 5% CO2, mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
The cells detach easily with PBS, but trypsin is necessary to disaggregate the cells
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
- Mol Cell Biol 1987;7:379-387 - PMID: 3031469
- Proc Natl Acad Sci USA 1993;90:8392-8396 - PMID: 7690960
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.