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ICLC catalog code: ATL95002
Continuous culture, grown as monolayer
SPECIES: mouse, Swiss albino
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Contact inhibited; transfection studies. The cell line is a valuable in vitro host system for oncogenic transformation studies. It is readily transformed by SV40 and polyoma viruses
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with G6PD, MD, NP)
Availability in cell line catalogues: DSMZ: ACC 173, IZSBS: BS CL 82
- J Cell Biol 1963;17:299-313 (reference paper)
- Virology 1965;27:179-185 - PMID: 4284655
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.