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ICLC catalog code: ATL99006
Continuous culture, grown as monolayer
SPECIES: mouse, 129
DEPOSITOR: Dr S. Astigiano, Transgenic Unit, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells undergo very limited differentiation under normal culture conditions, but can be induced to differentiate into a) parietal endoderm in the presence of retinoic acid (RA) and dibutyryl cyclic AMP; b) visceral endoderm when cultured in suspension as aggregates and treated with RA. Differentiation markers are: tissue type plasminogen activator (tPA) for parietal endoderm cells; alpha fetoprotein (AFP) and urokinase plasminogen activator (uPA) for visceral endoderm cells. In addition, both cell types lose the stage specific embryonic antigen-1 (SSEA-1), and synthesize large amounts of type IV collagen, laminin and fibronectin.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM (4.5 g/L glucose) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x10^4; 37C, 5% CO2 Passage: 6, mycoplasma negative, HOECHST and PCR
passage 6 after in vivo passage; split every two days; leave the cells with trypsin for one or two minutes at room temperature; the flasks must be presoaked with 0.1% gelatin for 10 minutes
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Availability in cell line catalogues: ECACC: 85061803, DSMZ: ACC 112
- Cell 1978;15:393-403 - PMID: 214238
- Proc Natl Acad Sci USA 1973;70:3899-3903 - PMID: 4521215
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.