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SPECIES: human, Caucasian
DSMZ catalog code: ACC 246
Morphology: cells grow in clusters in suspension
Description: human retinoblastoma established from the primary tumor (right eye) of a 2-year-old Caucasian girl in 1971 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS optimal split ratio at 1:2 two to three times per week; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 cell harvest of about 1.0-1.5 x 106 cells/ml;
doubling time of about 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype with 10% polyploidy; 47(45- 48)XX, +mar, der(4)t(4;?6)(p15;p21.2), add(12)(q24.32), add(15)(q23), add(20)(q13); large submetacentric marker with q-arm comprizing HSR) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +) * isoenzymes (confirmed as human with IEF of MDH, NP)
Availability in cell line catalogues: ATCC: HTB 18
- J Natl Cancer Inst 1974;53:347-360 - PMID: 4135597
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.